Spectroscopic techniques for study of phosphodiester bond formation by Escherichia coli RNA polymerase.
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چکیده
منابع مشابه
Transcript assisted phosphodiester bond hydrolysis by eukaryotic RNA polymerase II
Hydrolysis of the phosphodiester bonds of the transcript by bacterial RNA polymerase is assisted by 3′NMP of the RNA. Here we provide evidence that this mechanism is also involved in RNA cleavage by eukaryotic RNA polymerase II, suggesting that transcript assisted hydrolysis has emerged before divergence of bacteria and archaea/eukaryotes. Multisubunit RNA polymerase (RNAP), the enzyme accompli...
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RNA folding during transcription by Escherichia coli RNA polymerase analyzed by RNA self-cleavage.
We have used a self-cleaving RNA molecule related to a subsequence of plant viroids (a "hammerhead") to study the length-dependent folding of RNA produced during transcription by Escherichia coli RNA polymerase. Transcript elongation is arrested at defined positions using chain-terminating ribonucleoside triphosphate analogues (3'-deoxyNTP's or 3'-O-methylNTP's). When the transcript can form th...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)86430-x